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<td><h1><font color="#FFFFFF"><strong>Mark Hilge</strong><br>
<strong>Structural Biology<br>
Gallery</strong></font> </h1>
<h3><font color="#FFFFFF">Movies</font></h3>
<p><font color="#FFFFFF">Na+/K+ ATPase<br>
N-domain<br>
Rotation of the 25kD nucleotide binding domain of the Na+/K+ <br>
ATPase in complex with ATP. <br>
QuickTime Movie 270 x 300 Pixel<br>
DOWNLOAD N-domain.mov 1,7 MB </font></p>
<p><font color="#FFFFFF"><br>
11Å EM structure<br>
Glas body representation based on the EM map of the 11Å <br>
structure of the Na+/K+ ATPase fitted with the N-domain <br>
structure (red) determined by NMR and the X-ray structure of <br>
the A-, P-, and transmembrane domain (yellow) of the Ca2+ <br>
ATPase. The EM map was kindly rovided by Bill Rice & coworkers.<br>
QuickTime Movie 330 x 450 Pixel<br>
DOWNLOAD rotation3.mov 860 KB</font></p>
<p><font color="#FFFFFF"><br>
Pump cycle<br>
Molecular movie of the entire reaction cycle of the Na+/K+ <br>
ATPase. A detailed description can be found here.<br>
QuickTime Movie 400 x 384 Pixel<br>
DOWNLOAD pump-cycle-medium.mov 11,9 MB</font></p>
<p><font color="#FFFFFF"><br>
Na+/Ca2+ exchanger<br>
Hypothetical model<br>
Rotation of the hypothetical Na+/Ca2+ exchanger model that <br>
consists of a transmembrane domain (yellow), containing the <br>
Na+ and Ca2+ transport sites, and three regulatory domains <br>
located in the cytosol. Domains in red and green represent <br>
CBD1 and CBD2, respectively. Ca2+ binding and release events <br>
are relayed via the CLD (blue) to the ion-transport sites within
<br>
the transmembrane domain.<br>
QuickTime Movie 500 x 600 Pixel<br>
DOWNLOAD rot.-NCX.mov 3,7 MB</font></p>
<font color="#FFFFFF"> </font> <font color="#FFFFFF">Binding of Ca2+
to CBD1 and CBD2<br>
CBD1 is in red, CBD2 in green. Regions that undergo substantial <br>
conformational changes upon binding and release of Ca2+ are <br>
colored yellow and orange. From our NMR data we know that <br>
CBD1 Ca2+ binding sites in the absence of Ca2+ are largely <br>
unstructured. As the primary Ca2+ sensor CBD1 binds Ca2+ ions <br>
with affinities of around 100-400 nM. In contrast, conformational
<br>
changes in CBD2 are relatively minor.<br>
QuickTime Movie 334 x 400 Pixel<br>
DOWNLOAD H1+H2.mov 4,3 MB</font> <h3><font color="#FFFFFF">Covers</font></h3>
<p><font color="#FFFFFF">Molecular Cell<br>
22 (1), 2006<br>
Major players in Ca2+ homeostasis involve the Na+/Ca2+ <br>
exchanger, the plasma membrane and endoplasmic reticulum <br>
Ca2+ ATPases, the L-type Ca2+ channel DHPR as well as the <br>
ryanodine and IP3 receptors in the endoplasmic reticulum. <br>
With the exception of the Na+/Ca2+ exchanger all depicted <br>
transporters are either based on X-ray, NMR or EM structures. <br>
The mitochondrial Ca2+ uniporter and the mitochondrial Na+/Ca2+
<br>
exchanger are omitted due to lack of structural information. <br>
Artwork was created by Stephan Hilge. </font></p>
<p><font color="#FFFFFF">Ann N Y Acad Sci. Volume 1099, 2007<br>
Hypothetical model of the two major Ca2+ export systems, the <br>
Na+/Ca2+ exchanger (NCX) and the plasma membrane Ca2+ <br>
ATPase (PMCA). Artwork was created by Stephan Hilge.</font></p>
<h3><font color="#FFFFFF">Reprints</font></h3>
<p><font color="#FFFFFF">b-Mannanase<br>
Hilge M, Gloor SM, Rypniewski W, Sauer O, Heightman TD, <br>
Zimmermann W, Winterhalter K, Piontek K. High-<br>
resolution native and complex structures of thermostable <br>
b-mannanase from Thermomonospora fusca – substrate <br>
specificity in glycosyl hydrolase family 5. Structure 6, <br>
1433-44 (1998).<br>
DOWNLOAD Structure.pdf 1 MB</font></p>
<p><font color="#FFFFFF">Na+/K+ ATPase<br>
Hilge M, Siegal G, Vuister GW, Güntert P, Gloor SM, <br>
Abrahams JP. ATP-induced conformational changes of <br>
the nucleotide-binding domain of Na,K-ATPase. Nat Struct <br>
Biol. 10, 468-74 (2003).<br>
DOWNLOAD NSB.pdf 1,5 MB</font></p>
<p><font color="#FFFFFF">Na+/Ca2+ exchanger<br>
Hilge M, Aelen J, Vuister GW. Ca2+ regulation in the <br>
Na+/Ca2+ exchanger involves two markedly different <br>
Ca2+ sensors. Mol Cell. 22, 15-25 (2006).<br>
DOWNLOAD MC.pdf 828kB</font></p>
<p><font color="#FFFFFF">Hilge M, Aelen J, Perrakis A, Vuister GW.
Structural basis <br>
for Ca2+ regulation in the Na+/Ca2+ exchanger. Ann N Y <br>
Acad Sci. 1099, 7-15 (2007).<br>
DOWNLOAD NYAS.pdf 5,4 MB</font></p>
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